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M6518

Sigma-Aldrich

Macrophage Colony-Stimulating Factor human

M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

MCSF

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About This Item

MDL number:
MFCD00166591
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

200

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE and HPLC)

form

lyophilized powder

potency

0.500-5.000 ng/mL ED50

quality

endotoxin tested

mol wt

dimer 37 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.000 EU/μg

color

white

UniProt accession no.

P09603

storage temp.

−20°C

Gene Information

human ... CSF1(1435)

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General description

Macrophage Colony-Stimulating Factor (M-CSF) is a hematopoietic growth factor, which modulates the proliferation, differentiation and functional activation of monocytes. It is produced by fibroblasts, endothelial cells and bone marrow stromal cells. MCSF stimulates the functions of mature monocytes and macrophages.

Application

Macrophage Colony-Stimulating Factor human has been used for the differentiation of bone marrow-derived macrophages (BMDMs) and human monocyte-derived macrophages (MDMs). It has also been used for cell culture.

Biochem/physiol Actions

Macrophage colony-stimulating factor (M-CSF) augments many of the physiological functions of macrophages, and support osteoclast differentiation.

Other Notes

159 amino acids including N-terminal methionine (each monomer).

Physical form

Lyophilized from a sterile filtered buffered solution.

Analysis Note

The proliferative activity is tested in culture using the cytokine-dependent murine monocytic cell line M-NFS-60 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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