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M5634

Sigma-Aldrich

5-Mercuricytidine carbonate 5′-triphosphate triethylammonium salt

~90%

Synonym(s):

Hg-CTP

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About This Item

Linear Formula:
C9H15HgN3O14P3 · HCO3
CAS Number:
100940-62-3
Molecular Weight:
1148.52
MDL number:
MFCD00058366
UNSPSC Code:
41106305
PubChem Substance ID:
329818019
NACRES:
NA.51

Assay

~90%

form

solid

storage temp.

−20°C

SMILES string

CCN(CC)CC.NC1=NC(=O)N(C=C1[Hg])[C@@H]2O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]2O

InChI

1S/C9H15N3O14P3.C6H15N.Hg/c10-5-1-2-12(9(15)11-5)8-7(14)6(13)4(24-8)3-23-28(19,20)26-29(21,22)25-27(16,17)18;1-4-7(5-2)6-3;/h2,4,6-8,13-14H,3H2,(H,19,20)(H,21,22)(H2,10,11,15)(H2,16,17,18);4-6H2,1-3H3;/t4-,6-,7-,8-;;/m1../s1

InChI key

CKMVNPSLWQKVPG-WFIJOQBCSA-N

Application

5-Mercuricytidine carbonate 5;-triphosphate (Hg-CTP) may be used as a substrate to generate mercurated DNA that may be suitable for image based DNA sequencing strategies.

Signal Word

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - STOT RE 2

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Study of deoxyribonucleic acid replication in permeable cells of Bacillus subtilis using mercurated nucleotide substrates.
S Bhattacharya et al.
Biochemistry, 20(11), 3029-3034 (1981-05-26)
G Banfalvi et al.
Analytical biochemistry, 146(1), 64-70 (1985-04-01)
A method for isolating picomole quantities of nascent mercurated DNA from a mixture of cellular nucleic acids using affinity chromatography on thiol-agarose is described. Analysis of mercurated DNA (HgDNA) isolated in the presence of in vivo-labeled cellular RNA or in
A J Bridgman et al.
DNA sequence : the journal of DNA sequencing and mapping, 6(4), 199-209 (1996-01-01)
The development of high-resolution scanning-probe microscopes has reawakened interest in the possibility of sequencing large nucleic acid molecules by direct imaging. Such an approach would be facilitated by the availability of effective methods for increasing contrast by labelling specific nucleotides
Structural analyses on the mercury(II)-mediated T-T base pair.
Tanaka Y, Oda S, et al.
Nucleic Acids Symposium Series, 50, 47-48 (2006)
S Bhattacharya et al.
The Journal of biological chemistry, 257(4), 1610-1612 (1982-02-25)
Mercurated DNA was synthesized in permeable cells of Bacillus subtilis, using 5-mercurideoxycytidine triphosphate as one of the substrates, and was separated from parental unsubstituted DNA by isopycnic centrifugation in CsCl gradients. The ability of mercurated DNA to transform auxotrophic strains
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