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Key Documents

L7901

Sigma-Aldrich

Monoclonal Anti-LEF-1 (HMG DNA Binding Domain) antibody produced in mouse

~1 mg/mL, clone REMB6, purified immunoglobulin

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

REMB6, monoclonal

mol wt

antigen 55-57 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

western blot: 1-5 μg/mL using whole extracts of human Jurkat cells or transiently expressed human LEF-1 in whole cell lysates from Cos-1 cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... LEF1(51176)

Immunogen

full length recombinant LEF-1.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.5, containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marloes L de Groote et al.
International journal of cancer, 134(2), 280-290 (2013-07-09)
Ovarian cancer is a difficult-to-treat cancer with a 5-year survival rate of only ∼45%, due to late diagnosis and therapy resistance. In need of new therapeutic approaches, induction of intercellular adhesion molecule (ICAM)-1 expression might be of interest, since the
Elisabetta Lambertini et al.
BMC molecular biology, 11, 13-13 (2010-02-05)
Lymphoid Enhancer Factor-1 (Lef-1) is a member of a transcription factor family that acts as downstream mediator of the Wnt/beta-catenin signalling pathway which plays a critical role in osteoblast proliferation and differentiation. In a search for Lef-1 responsive genes in

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