I6277
Anti-Interleukin-2 Soluble Receptor β antibody produced in goat
affinity isolated antibody, lyophilized powder
Synonym(s):
Anti-IL-2 sRβ
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About This Item
MDL number:
UNSPSC Code:
51111800
biological source
goat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
technique(s)
indirect ELISA: suitable
neutralization: suitable
western blot: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... IL2RB(3560)
General description
Interleukin-2 (IL-2) is a cytokine produced by activated T cells in response to antigen stimulation. IL-2 is important for proliferation of lymphocytes, natural killer cells and macrophages and in the differentiation of CD4+ cells into Th1 and Th2 effector cells. The effects of IL-2 are mediated by the signalling activated by its binding to IL-2 receptor (IL-2 R). IL-2 R which contains three membrane subunits: α, β and γ. The β and the γ subunits complex results in the binding of IL-2 with higher affinity. IL-2 R β is expressed on resting monocytes and B cells, CD8+ and CD4+T cells, NK cells and neutrophils and mature thymocytes. IL-2 R activates key signalling pathways such as MAPK, JAK/STAT as well as PI3K/Akt. Activation of these pathways modulates production of inflammatory cytokines, survival and progression through cell cycle
Anti-Human IL-2 soluble receptor β (IL-2 sRβ) specifically reacts with human IL-2 Rβ. The antibody shows less than 15% cross reactivity with recombinant human IL-3 sRα, less than 5% cross reactivity with recombinant human IL-1 RII, IL-2 sRγ, IL-5 sRα, and less than 2% cross reactivity with recombinant human IL-2 sRα, IL-4 sR, and IL-5sRβ. In addition, the antibody shows no cross reactivity with other cytokines tested.
Anti-Human IL-2 soluble receptor β (IL-2 sRβ) specifically reacts with human IL-2 Rβ. The antibody shows less than 15% cross reactivity with recombinant human IL-3 sRα, less than 5% cross reactivity with recombinant human IL-1 RII, IL-2 sRγ, IL-5 sRα, and less than 2% cross reactivity with recombinant human IL-2 sRα, IL-4 sR, and IL-5sRβ. In addition, the antibody shows no cross reactivity with other cytokines tested.
Specificity
The antibody will neutralize human cell surface IL-2 receptor β mediated-bioactivity. For cells expressing high affinity IL-2 receptors, use of both anti-IL-2 Rα and anti-IL-2 Rβ is recommended. The antibody shows ≤15% cross-reactivity with recombinant human IL-3 sRα, ≤5% cross-reactivity with recombinant human IL-1 RII, IL-2 sRγ, IL-5 sRα, and ≤2% cross-reactivity with recombinant human IL-2 sRα, IL-4 sR, and IL-5 sRβ.
Immunogen
recombinant human IL-2 soluble receptor β (IL-2 sRβ) expressed in Sf 21 insect cells.
Application
Anti-Human IL-2 soluble receptor β (IL-2 sRβ) antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For indirect ELISA, the recommended concentration is 0.5-1.0 μg/ml. The antibody is suitable for IL-2 sRβ neutralization reactions at a working antibody concentration of 10-30 μg/ml.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Matthew A Williams et al.
Nature, 441(7095), 890-893 (2006-06-17)
Although interleukin-2 (IL-2) was initially characterized as the primary T-cell growth factor following in vitro activation, less is known about its role in shaping T-cell responses to acute infections in vivo. The use of IL-2- or IL-2-receptor-deficient mice is problematic
B H Nelson et al.
Advances in immunology, 70, 1-81 (1998-10-02)
Studies of the biology of the IL-2 receptor have played a major part in establishing several of the fundamental principles that govern our current understanding of immunology. Chief among these is the contribution made by lymphokines to regulation of the
Katrina K Hoyer et al.
Immunological reviews, 226, 19-28 (2009-01-24)
Interleukin-2 (IL-2) has multiple, sometimes opposing, functions during an inflammatory response. It is a potent inducer of T-cell proliferation and T-helper 1 (Th1) and Th2 effector T-cell differentiation and provides T cells with a long-lasting competitive advantage resulting in the
Hans Dooms et al.
Journal of immunology (Baltimore, Md. : 1950), 172(10), 5973-5979 (2004-05-07)
The acquisition of long-term survival potential by activated T lymphocytes is essential to ensure the successful development of a memory population in the competitive environment of the lymphoid system. The factors that grant competitiveness for survival to primed T cells
Arian Laurence et al.
Immunity, 26(3), 371-381 (2007-03-17)
Recent work has identified a new subset of effector T cells that produces interleukin (IL)-17 known as T helper 17 (Th17) cells, which is involved in the pathophysiology of inflammatory diseases and is thought to be developmentally related to regulatory
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