Before lyophilyzation, ficoll & sucrose are added stabilizers & the hemoglobin is dialyzed against 25 mM ammonium bicarbonate containing 0.1 mM EDTA. It is expected that after lyophilization, there should be virtually no or substantially reduced ammonium bicarbonate. Stabilizers are the major components of the final product.
H0267
Hemoglobin A0, Ferrous Stabilized human
lyophilized powder
Synonym(s):
Hemoglobin A0 Ferrous Stabilized, Hemoglobin-A0
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
eCl@ss:
42030116
NACRES:
NA.61
Pricing and availability is not currently available.
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biological source
human
Quality Level
Assay
97-100% (agarose gel electrophoresis)
form
lyophilized powder
technique(s)
immunofluorescence: suitable
solubility
H2O: soluble 20 mg/mL
suitability
suitable for electrophoresis and chromatography standard
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... HBB(3043)
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General description
Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide.
has not been tested for functional equivalence against native preparations (unlyophilized ferrous hemoglobins).
Application
Hemoglobin A0, ferrous stabilized human has been used:
- as the non-fluorescent absorber in the preparation of tissue-mimicking phantoms[1]
- to see how coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype[2]
- to study the interference of hemoglobin (Hb) A0, HbA2, and HbS in the assay of HbF[3]
Biochem/physiol Actions
Variation in the haemoglobin (Hb) encoding genes results in haemoglobinopathies such as thalassaemia and atypical structural variants like Hb Lepore and HbE.[5]
Packaging
Package size indicates the amount of hemoglobin as determined by the procedure of Drabkin, D.L., J. Biol. Chem., 164, 703 (1946).
Reconstitution
When reconstituted with buffer, gives >85% ferrous hemoglobin.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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From which buffer was Product H0267 lyophilized? Did this buffer contain primary amines?
1 answer-
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Does the product, H0267, contain HbA1c?
1 answer-
The glycation level of this product, i.e., HbA1c, has not been characterized.
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How can Product H0267, Hemoglobin A0, be dissolved?
1 answer-
Hemoglobin is soluble in water at 20 mg/mL. Solutions in phosphate buffer at about neutral pH can also be prepared.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solution stability of Product H0267, Hemoglobin A0?
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Solutions of ferrous hemoglobin are not stable indefinitely due to the likely autoxidation of the heme group leading to the formation of the ferric derivative (methemoglobin). It is recommended to prepare solutions fresh in deaerated water or buffer. If necessary, store such prepared solutions no more than 1-2 days at 2-8°C.
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What is the structure of Product H0267, Hemoglobin A0?
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Hemoglobin A0 is the major hemoglobin protein of adult hemoglobin. It is a tetrameric protein consisting of 2 alpha and 2 beta chains, each of which contains a heme moiety and iron.
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Is there methemoglobin in Product H0267, Hemoglobin A0?
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The product may contain a small amount of methemoglobin as described on the specification sheet.
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Can Product H0267, Hemoglobin A0, be used in functional studies involving the binding and release of oxygen as a ligand?
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Sigma has not validated this hemoglobin for use in functional studies. The iron in the heme moiety is in the +2 state. However, this does not mean that this hemoglobin will behave necessarily like native hemoglobin nor be useful for functional studies of the molecule.
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What is the molecular weight of H0267 Hemoglobin A0?
1 answer-
Sigma has not evaluated the molecular weight of hemoglobin. Based on the reported sequence and including the weight of the 4 heme groups and 4 iron atoms, the molecular weight is approximately 64,677 Daltons. The Structure and Action of Proteins, p. 52, Harper and Row (1969).
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