GE17-1128-01

MAbtrap^® Kit

Cytiva 17-1128-01, pkg of 1 kit

• UNSPSC Code: 13111000
• NACRES: NA.56

Properties

• ligand: recombinant protein G lacking albumin-binding region
• packaging: pkg of 1 kit
• manufacturer/tradename: Cytiva 17-1128-01
• storage condition: (20% Ethanol)
• parameter: <4 mL/min flow rate; 42 psi
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 7 mm
• matrix: highly cross-linked 6% agarose
• avg. part. size: 34 μm
• cleaning: 2-9
• working range: 3-9
• capacity: >1 mg binding capacity (lgG/column)
• storage temp.: 2-8°C

Description

General description

MAbTrap^® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

MAbTrap^® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap^® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap^® column, prepacked with Protein G Sepharose^™ High Performance.

Features and Benefits

• Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
• Kit contains one HiTrap^® Protein G HP column prepacked with Protein G Sepharose^™ High Performance and enough buffer concentrate for 20 preparations with a syringe.
• MAbTrap^® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

MAbTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H226,H319,H315
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids