Generally, larger particles offer faster flow rates than finer particles, but poorer resolution.
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Quality Level
form
slurry
technique(s)
LPLC: suitable
matrix
Agarose
matrix active group
polymer
swelling
1 g swells to 15-20 mL gel
bead size
40-120 μm
separation technique
size exclusion (SEC)
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General description
Sephadex® is composed of repeating glucose units attached by α-1,6 glucosidic bonds. This structure ensures more aptamer binding sites and greater binding capacity.[1] It is suitable for large-scale purification and is considered stable and can be utilized more than once.
Fractionation Range (MW)
Globular Proteins: 4,000 - 150,000
Dextrans: 1,000 - 100,000
Fractionation Range (MW)
Globular Proteins: 4,000 - 150,000
Dextrans: 1,000 - 100,000
Application
Sephadex®G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography
It has been used:
It has been used:
Other Notes
G100120-100G′s updated product number is GE17-0060-01
Legal Information
Sephadex is a registered trademark of Cytiva
replaced by
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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How do I determine which size to use for Product G100120, Sephadex® G-100 columns?
1 answer-
Helpful?
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What is the maximum flow rate for Product G100120, Sephadex® G-100 columns?
1 answer-
The maximum flow rate is 4.2 ml/min. Data are based on columns of 2.5 cm diameter with a bed height of 30 cm.
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My Sephadex® G-100 column is cracking. What is the maximum operating pressure that Product G100120 can take?
1 answer-
The maximum operating pressure for this gel is 96 cm water = 1.6 psi.
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