F4892
D-Fructose Dehydrogenase from Gluconobacter sp.
≥20 units/mg solid
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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form
amorphous solid
specific activity
≥20 units/mg solid
mol wt
~140 kDa
shipped in
wet ice
storage temp.
−20°C
Application
D-Fructose Dehydrogenase from Gluconobacter sp has been used as a standard in the carbohydrate binding specificity assay and SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis).
This enzyme is also used in a number of basic research projects to examine the electrochemical properties of enzyme-catalyzed electrode reactions called bioelectrocatalysis.This enzyme is useful for enzymatic determination of D-fructose in clinical analysis.
Biochem/physiol Actions
D-fructose dehydrogenase is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca. 140 kDa, consisting of subunits I (67kDa), II (51 kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively.
Physical properties
Isoelectric point : 5.0 ± 0.1
Michaelis constant : 5 x 10‾3M (D-Fructose)
Inhibitors : Ag+, Hg++, SDS
Optimum pH : 4.0
Optimum temperature : 37°C
pH Stability : pH 4.0 – 6.0 (25°C, 16hr)
Thermal stability : Below 40°C (pH 4.5, 15min)
Michaelis constant : 5 x 10‾3M (D-Fructose)
Inhibitors : Ag+, Hg++, SDS
Optimum pH : 4.0
Optimum temperature : 37°C
pH Stability : pH 4.0 – 6.0 (25°C, 16hr)
Thermal stability : Below 40°C (pH 4.5, 15min)
Unit Definition
One unit will convert 1.0 μmole D-fructose to 5-ketofructose per min at pH 4.5 at 37 °C.
Physical form
This product is supplied as an amorphous solid containing ~ 80% stabilizers, sugars, amino acids and BSA.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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