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E8029

Sigma-Aldrich

E-Toxate™ Endotoxin standard

for endotoxin quantitation

Synonym(s):

E. coli 0.55:B5 LPS standard

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.24
Pricing and availability is not currently available.

Quality Level

200

contains

solubilizer

storage temp.

2-8°C

Application

E-Toxate endotoxin standard was used along with the limulus amebocyte lysate assay kit to determine the concentration of endotoxins in isolated samples of human holotransferrin and apotransferrin used to treat primary hepatocyte cultures. [1]

Other Notes

A preparation of E. coli 055:B5 lipopolysaccharide
Can be stored at 2-8 °C when stored separately from the E-Toxate kit.

Analysis Note

Standardized against USP reference standard endotoxin (RSE)

Legal Information

E-Toxate is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000331446
0000269511
0000291838
0000291057
SLCJ0557

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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Akrivi Chrysanthopoulou et al.
Clinical immunology (Orlando, Fla.), 247, 109240-109240 (2023-01-25)
Kruppel-like factor 2 (KLF2) has been linked with fibrosis and neutrophil-associated thromboinflammation; however, its role in COVID-19 remains elusive. We investigated the effect of disease microenvironment on the fibrotic potential of human lung fibroblasts (LFs) and its association with KLF2
Anna Scanu et al.
International journal of molecular sciences, 23(21) (2022-11-12)
We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation. In CIA, mice were intraperitoneally injected with PBS, HA, or BPI plus or minus
Lan Lin et al.
Blood, 110(6), 2182-2189 (2007-06-02)
The peptide hormone hepcidin is the principal regulator of systemic iron homeostasis. We examined the pathway by which iron stimulates the production of hepcidin. In humans who ingested 65 mg of iron, the increase in transferrin saturation preceded by hours
Akrivi Chrysanthopoulou et al.
JCI insight, 6(18) (2021-07-30)
Innate immunity and chronic inflammation are involved in atherosclerosis and atherothrombosis, leading to target organ damage in essential hypertension (EH). However, the role of neutrophils in EH is still elusive. We investigated the association between angiotensin II (Ang II) and

Questions

1–6 of 6 Questions  

  1. Hi, For E8029 endotoxin quantification product, 1. Is it a powder or liquid? 2. Solubility in water if it's a powder? How much water is needed to prepare a solution? Thanks,

    1 answer

    1. This product is a lyophilized powder typically used as a standard in the E-Toxate Endotoxin kits. See the link below to review these kits. For use with these kits, the material is reconstituted in endotoxin-free water at 4000UE/mL. Each vial contains a range of 10,000 - 20,000 units which is reported in the lot specific Certificate of Analysis. The mass weight is not determined. However, the amount of powder may be estimated based on the conversion factor of 5 EU per nanogram.
      Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/e8029#product-documentation

      Please see the link below to review the E-Toxate Endotoxin Kit products:
      https://www.sigmaaldrich.com/substance/etoxatekit1234598765

      Please see the link below to review the kit protocol with reconstitution instructions for this standard:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/378/800/et0200-pis-ms.pdf

      Helpful?

  2. What is the amount of the powder in each vial in grams or milligrams?

    1 answer

    1. The product comes as 10000 - 20000 EU Endotoxin per vial. Unfortunately, the exact weight information is not available.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. How is the E-Toxate™ Endotoxin standard, Product E8029, dissolved?

    1 answer

    1. E8029 is reconstituted in endotoxin-free water to a concentration of 4000 EU/mL.  Reconstituted standard solutions can be stored at 2 - 8°C for at least 2 weeks.

      Helpful?

  5. How much water should I use to reconstitute my E-Toxate™ Endotoxin standard, Product E8029?

    1 answer

    1. Each batch of endotoxin standard varies slightly in its activity so the amount of water to use may vary from lot to lot.  The certificate of analysis will give the amount of endotoxin units in the vial, so that the end user may calculate the appropriate amount of water to add to the vial in order to reconsitute to a final concentration of 4000 EU/mL.  The certificate of analysis for each lot may be retrieved from our website.

      Helpful?

  6. What is the E-Toxate™ Endotoxin standard, Product E8029?

    1 answer

    1. The endotoxin standard is purified from a crude extract of lipopolysaccharide from E. coli 055:B5 and standardized against the current USP reference standard.  It is reconstituted with an appropriate amount of water to yield a 4000 EU/mL solution.

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