MM466
12070507, human skin (metastasis), Epithelial
Synonym(s):
MM 466
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About This Item
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product name
MM466, 12070507
biological source
human skin (metastasis)
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
receptors
Not specified
relevant disease(s)
metastasis
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Cell Line Origin
Human melanoma metastatic site lymph node
Cell Line Description
mM466 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1353.
DNA Profile
Not specified
Culture Medium
RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.
Subculture Routine
Optimal split ratio 1:2-1:4 (seeding density 1.4 x 104 cells/cm2). Cells incubated at 37 °C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Medium change twice weekly. The cell line MM466 may take up to 72 hours to recover from thaw.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Peter Parsons - Queensland Institute of Medical Research, Australia
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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