TKE2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 11033107, mouse cornea. Epithelial cells isolated from basal limbal layer.

• Synonym(s): TKE, TKE-2
• UNSPSC Code: 41106514

Properties

• biological source: eye; mouse (CD-1)
• packaging: tube of 5 μg 11033107-DNA-5UG; pkg of vial of cells 11033107-1VL
• gender: female
• growth mode: Adherent
• karyotype: Normal karyotype of the female mouse CD1 strain
• morphology: Epithelial; small and regular cobblestone-like shape
• products: Stains positive for nuclear p63 expression and K14 keratin.
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice

Description

Cell Line Origin

Mouse cornea epithelial progenitor cells

Cell Line Description

TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.

DNA Profile

Not specified

Culture Medium

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 2 x 10² - 2 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

Other Notes

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