54SCC3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: Mouse x Rat Hybridoma nerve
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Modal No. varied with passage see reference
• Morphology: oligopolar
• Manufacturer/tradename: ECACC

Properties

• biological source: Mouse x Rat Hybridoma nerve
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 08062561-DNA-5UG, pkg of vial of cells 08062561-1VL
• growth mode: Adherent
• karyotype: Modal No. varied with passage see reference
• morphology: oligopolar
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Biochem/physiol Actions

Hybrid generated by fusion of bromodeoxyuridine-resistant mutant of rat glioma cell line C6 (C6-BU-1, Sigma Catalogue number 08062563) and the 6-thioguanine-resistant mouse L cell fibroblast line A9 (Sigma Catalogue number 08062526) using inactivated Sendai virus. Cells formed from such a fusion are multipolar and extend small processes.

Rat glioma x mouse L cell fibroblast hybrid

Preparation Note

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.