C9499
Cellulase, thermostable from Clostridium thermocellum
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym(s):
1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
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Recommended Products
recombinant
expressed in E. coli
Quality Level
Assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥40 units/mg protein
mol wt
41 kDa
concentration
19-21 mg protein/mL (UV)
shipped in
wet ice
storage temp.
2-8°C
Application
Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries [1]. Cellulase is added to detergents to improve cleansing properties [1]. Cellulase, product C9499, is from Clostridium thermocellum and is recombinant and expressed in E. coli.
Biochem/physiol Actions
Cellulases are produced by the intestinal flagellates of termites which are important for cellulose digestion of their host termites [2]. Cellulases have different microbial origins and therefore different protein sequences [1]. Product C9499 exhibits endo-cellulase, β-glucanase activity when assayed using insoluble AZCL-linked substrates as well as exo-cellulase and cellobiohydralase activities.
Unit Definition
One unit will produce 1 μmole of reducing sugar (measured as glucose) from β-glucan per minute at pH 5.8 at 70 °C.
Physical form
Supplied as as solution in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 25% glycerol.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Gaku Tokuda et al.
Biology letters, 3(3), 336-339 (2007-03-22)
The intestinal flagellates of termites produce cellulases that contribute to cellulose digestion of their host termites. However, 75% of all termite species do not harbour the cellulolytic flagellates; the endogenous cellulase secreted from the midgut tissue has been considered a
A Brant et al.
Occupational and environmental medicine, 61(9), 793-795 (2004-08-20)
Three employees from two different detergent companies were investigated for occupational asthma, using skin prick tests, serum specific IgE, and specific bronchial challenge. Two were challenged with lipase and one with cellulase. All three cases had immunological evidence of sensitisation
Wei Weiqi et al.
Bioresource technology, 128, 725-730 (2012-12-25)
Combination of liquid hot water pretreatment (LHWP) and wet disk milling (WDM) was investigated in this study to enhance the sugar recovery yield both in prehydrolyzate and enzymatic hydrolyzate. The results show that WDM with LHWP at 180 °C for
Antonella Amore et al.
Microbial cell factories, 11, 164-164 (2012-12-27)
The use of lignocellulosic materials for second generation ethanol production would give several advantages such as minimizing the conflict between land use for food and fuel production, providing less expensive raw materials than conventional agricultural feedstock, allowing lower greenhouse gas
Aditya Bhalla et al.
Bioresource technology, 128, 751-759 (2012-12-19)
Second-generation feedstock, especially nonfood lignocellulosic biomass is a potential source for biofuel production. Cost-intensive physical, chemical, biological pretreatment operations and slow enzymatic hydrolysis make the overall process of lignocellulosic conversion into biofuels less economical than available fossil fuels. Lignocellulose conversions
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