C7740

Anti-phospho-α-B Crystallin (14-25) (pSer¹⁹) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD07370435
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, IHC (p), WB
• Citations: 6

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: rat, bovine, human, mouse
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10 μg/mL, indirect immunofluorescence: 5 μg/mL, western blot: 0.5 μg/mL using human cell extract
• UniProt accession no.: P02511
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer19)
• Gene Information: human ... CRYAB(1410) ; mouse ... Cryab(12955) ; rat ... Cryab(25420)

Description

Immunogen

synthetic phosphopeptide surrounding the region of human α-B crystallin that contains serine 19.

Application

Anti-phospho-α-B Crystallin (14-25) (pSer¹⁹) antibody produced in rabbit

• Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 10μg/mL
• Indirect immunofluorescence at a concentration of 5μg/mL
• Western blotting at a concentration of 0.5μg/mL using human cell extract
• It also used in 1- and 2-D immune blotting analysis.

Biochem/physiol Actions

α-Crystallin B chain is a protein encoded by the CRYAB gene in humans. αB-crystallin (ABC) and HSP27 are most abundant in human tissues. It is predominantly expressed in triple-negative breast cancer (TNBC) and leads to aggressive tumor phenotype. αB-crystallin expression is primary found in breast carcinomas and brain metastases. αB-crystallin is overexpressed or silenced in two different TNBC cell lines. It is a novel regulator of brain metastasis in TNBC and acts as a potential biomarker for drug target in aggressive disease. αB-crystallin and VEGF expressions in human conjunctival squamous cell carcinoma (CSCCs) may play an important role in the pathogenesis. Mutation in the gene is responsible for a number of inherited human disorders, which include cardiomyopathy, skeletal muscle myopathy and cataracts.

Does not detect the unphosphorylated protein.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 3% bovine serum albumin and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable