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C-41330

Sigma-Aldrich

Macrophage Detachment Solution DXF

250 ml

Synonym(s):

Macrophage Detachment Solution

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

aqueous solution

packaging

pkg of 250 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Macrophage Detachment Solution DXF

Application

Macrophages are strongly adherent and are not effectively dislodged from culture surfaces by standard enzymatic cell dissociation reagents. Additionally, these cells are highly sensitive to improper dissociation procedures. The Macrophage Detachment Solution DXF is non-enzymatic, defined and animal component-free. It was especially designed for the gentle release of adherent macrophages and guarantees the best possible cell viability, even after prolonged exposure times. Unlike enzyme-based solutions, the Macrophage Detachment Solution DXF does not alter cell surface proteins and neutralization is not necessary.Note: The Macrophage Detachment Solution DXF is not suitable for the dissociation of other adherent cell types.

Quality

All lots of PromoCell Macrophage Detachment Solution DXF are subjected to comprehensive quality control tests. Each lot is routinely tested for biological function and absence of cytotoxicity. Approved in-house lots are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store at 2 to 8°C immediately after arrival. If stored properly, the product is stable until the expiry date stated on the label.

Subculture Routine

Click here for more information.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Hematopoietic Stem & Immune Cell Culture

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Hematopoietic Stem & Immune Cell Culture

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Hematopoietic Stem & Immune Cell Culture

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Hematopoietic Stem & Immune Cell Culture

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Protocols

In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

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