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C2900

Sigma-Aldrich

Chloramphenicol Acetyltransferase from Escherichia coli

lyophilized powder, 50,000-150,000 units/mg protein (Lowry)

Synonym(s):

Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT

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About This Item

CAS Number:
9040-07-7
Enzyme Commission number:
2.3.1.28 (BRENDA, IUBMB)
MDL number:
MFCD00130772
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

50,000-150,000 units/mg protein (Lowry)

mol wt

75 kDa (three identical subunits)

shipped in

dry ice

storage temp.

−20°C

Gene Information

Escherichia coli ... cat(2847485)

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General description

The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.

Biochem/physiol Actions

The enzyme responsible for chloramphenicol resistance in bacteria. Catalyzes the conversion of acetyl-CoA + chloramphenicol to CoA + chloramphenicol 3-acetate.

Unit Definition

One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25 °C.

Physical form

Partially purified; contains Tris buffer salts.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLBF8205V
125H6826
099F68255
099F68253
075K86191

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Ziyaad Valley-Omar et al.
Virology journal, 8, 462-462 (2011-10-07)
HIV-1 Gag virus like particles (VLPs) used as candidate vaccines are regarded as inert particles as they contain no replicative nucleic acid, although they do encapsidate cellular RNAs. During HIV-1 Gag VLP production in baculovirus-based expression systems, VLPs incorporate the
Tuija A A Pöyry et al.
Journal of virology, 85(19), 10178-10188 (2011-08-05)
Translation initiation dependent on the foot-and-mouth disease virus (FMDV) internal ribosome entry site (IRES) occurs at two sites (Lab and Lb), 84 nucleotides (nt) apart. In vitro translation of an mRNA comprising the IRES and Lab-Lb intervening segment fused to
Junbo Liu et al.
FEBS letters, 586(12), 1719-1723 (2012-05-16)
Bicoid (Bcd) is a Drosophila morphogenetic protein and a transcriptional activator. Genetic studies have suggested a role of sumoylation in Bcd function, but it is unknown how Bcd activity is affected specifically by its own sumoylation status. Here we show
Bédis Dridi et al.
The Journal of antimicrobial chemotherapy, 66(9), 2038-2044 (2011-06-18)
Methanogenic archaea are constant members of the human oral and digestive microbiota retrieved, in particular, from periodontitis lesions. The objective of the study was to determine their susceptibility to antimicrobials. Using the macrodilution method in Hungate tubes with optical microscope
Fang Wang et al.
Gene, 500(1), 59-62 (2012-04-05)
Numerous matrix attachment regions (MARs) have been used to improve transgene expression in genetic engineering, but an efficient and stable expression vector is lacking. In the present study, a vector named pCCF containing chloramphenicol acetyltransferase (CAT) reporter gene cassettes was
View All Related Papers

Protocols

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

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