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C100

Sigma-Aldrich

Catalase from bovine liver

aqueous suspension, 40,000-60,000 units/mg protein (E1%/405)

Synonym(s):

H2O2:H2O2 oxidoreductase

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About This Item

CAS Number:
9001-05-2
Enzyme Commission number:
1.11.1.6 (BRENDA, IUBMB)
MDL number:
MFCD00081483
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Pricing and availability is not currently available.

form

aqueous suspension

Quality Level

200

specific activity

40,000-60,000 units/mg protein (E1%/405)

mol wt

tetramer ~250 kDa

isoelectric point

5.4

UniProt accession no.

P00432

shipped in

wet ice

storage temp.

2-8°C

SMILES string

O(CC)C(=O)c1ccc(cc1)O

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

InChI key

NUVBSKCKDOMJSU-UHFFFAOYSA-N

Gene Information

cow ... CAT(280743)

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General description

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Application

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver may be used:

  • to prepare H2O2-O2 based biocathode for applications in glucose biofuel cells
  • to study the kinetic properties and storage stability of catalase immobilized on to florisil
  • in glutathione-mediated superoxide generation in an aqueous solution

Biochem/physiol Actions

Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.

Caution

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Unit Definition

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Preparation Note

This product is offered as a catalase suspension in water containing 0.1% (w/v) thymol with activity of 40,000-60,000 units/mg protein. To remove the thymol preservative, the catalase crystals may be pelleted by centrifugation, the supernatant, discarded, the pellet resuspended in water, and then pelleted again. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCP4294
SLCG5609
SLCH7352
SLCC3864
SLBS1139V

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Shi-Ying Cai et al.
Cellular and molecular gastroenterology and hepatology, 9(4), 679-688 (2019-12-31)
Inflammation plays an important role in the pathogenesis of cholestatic liver injury, but it is unclear whether the inflammasome is involved and is the objective of this study. Gene expression was analyzed in the livers of patients with primary biliary
Thomas L Kukar et al.
Nature, 453(7197), 925-929 (2008-06-13)
Selective lowering of Abeta42 levels (the 42-residue isoform of the amyloid-beta peptide) with small-molecule gamma-secretase modulators (GSMs), such as some non-steroidal anti-inflammatory drugs, is a promising therapeutic approach for Alzheimer's disease. To identify the target of these agents we developed
Ruby Peters et al.
Scientific reports, 8(1), 10418-10418 (2018-07-12)
Single molecule localization microscopy (SMLM) methods produce data in the form of a spatial point pattern (SPP) of all localized emitters. Whilst numerous tools exist to quantify molecular clustering in SPP data, the analysis of fibrous structures has remained understudied.
Brian J McHugh et al.
PLoS pathogens, 15(4), e1007694-e1007694 (2019-04-13)
Pulmonary infections are a major global cause of morbidity, exacerbated by an increasing threat from antibiotic-resistant pathogens. In this context, therapeutic interventions aimed at protectively modulating host responses, to enhance defence against infection, take on ever greater significance. Pseudomonas aeruginosa
Lorena Betancor et al.
Biotechnology progress, 19(3), 763-767 (2003-06-07)
Bovine liver catalase was immobilized on different supports. The tetrameric nature of this enzyme was found to cause its rapid inactivation in diluted conditions due to subunit dissociation, a fact that may rule out its industrial use. Multi-subunit immobilization using
View All Related Papers

Protocols

Enzymatic Assay of Catalase (EC 1.11.1.6)

This procedure may be used for all Catalase products.

Questions

1–7 of 7 Questions  

  1. Hello, For how long is the bovine catalase stable in aqueous form ? and as a lyophilized powder ? Thank you

    1 answer

    1. This product is supplied in the form of aqueous suspension which is quite stable when stored refrigerated at 2 - 8 °C. Avoid freezing the solution as the thawing process will cause a decline in activity. This item is not assigned an expiration date, but is warrantied for 1 year from the date of shipment. A lyophilized Catalase product, C40, is assigned a retest date of 3 years from the date of release. See the links below for additional information.

      Product Data Sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/338/431/c100pis.pdf

      Product Dating Information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/190/408/product-dating-information-ms.pdf

      C40 Catalase, lyophilized powder, specification sheet:
      https://www.sigmaaldrich.com/specification-sheets/253/868/C40-BULK__________SIGMA____.pdf

      Helpful?

  2. Your website shows that Product No. C100 is catalase. But I have some notes that indicate that C100 is your product number for calmidazolium chloride. Which one is right?

    1 answer

    1. C-100 was used as a product number for calmidazolium chloride until about 1999 by Research Biochemicals International (RBI), which is now part of Sigma-Aldrich. Calmidazolium chloride is currently listed under Sigma Product No. C3930. So now we use the C100 only for catalase.

      Helpful?

  3. Can Product C100, Catalase from bovine liver, be shipped on wet ice or dry ice?

    1 answer

    1. Product No. C100 normally ships at ambient temperature. If desired, it can be shipped on wet ice upon special request. C100 cannot be shipped on dry ice. That is, the suspension can be harmed by freezing.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Is Product C100, Catalase from bovine liver, sterile?

    1 answer

    1. C100 is not necessarily sterile. But it does contain a preservative (0.1% thymol), which should prevent microbial growth.

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  6. Is Product C100, Catalase from bovine liver, a solution or a suspension?

    1 answer

    1. Most of the protein (catalase) in Product No. C100 is a solid in suspension, with a small percentage actually in solution.

      Helpful?

  7. What is the catalase concentration of Product C100, Catalase from bovine liver?

    1 answer

    1. There is no "official" specification, but the lot history shows that it has varied from 15-100 mg/mL. The concentration is provided on the certificate of analysis for each lot. Note that most of the catalase is typically a solid in suspension, with a small percentage actually in solution.

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