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Cathepsin B from human placenta

Name: Cathepsin B from human placenta
Brand: SIGMA

lyophilized powder, ≥5 units/mg protein

Synonym(s):

Cathepsin B₁

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About This Item

CAS Number:

9047-22-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.22.1 (BRENDA, IUBMB)

MDL number:

MFCD00130748

Specific activity:

≥5 units/mg protein

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Properties

form

lyophilized powder

Quality Segment

200

specific activity

≥5 units/mg protein

mol wt

24.5 kDa

composition

Protein, ~50% Lowry

solubility

H₂O: soluble 1.0 mg/mL, clear

UniProt accession no.

P07858

storage temp.

−20°C

Gene Information

human ... CTSB(1508)

Description

General description

Cathepsin B is encoded by the gene CTSB and is located in the human chromosome at 8p23.1. Cathepsin B is a lysosomal proteolytic enzyme of cysteine protease family. It is present in all mammalian cells. It is essential for the intracellular protein turnover.

Application

Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer′s research, as it may have a role in the natural defense against the disease . Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells.

Cathepsin B from human placenta has been used in the in vitro digestion of modified myelin oligodendrocyte glycoprotein (MOG). It has also been used in determining the effect of cysteine protease inhibitors.

Biochem/physiol Actions

Cathepsin B preferentially cleaves at the caboxyl side of Arg-Arg bonds in small molecule substrates. Cathepsin B has been shown to degrade β-amyloid precursor protein into harmless fragments . Cathepsin B inhibition has been shown to limit bone metastasis in breast cancer .

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Physical form

Lyophilized powder containing phosphate buffer salts

Preparation Note

Dissolves in water at 1 mg/mL concentration, yieliding a clear solution.

Other Notes

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

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This Item	C8571	SAB5702092	C6243
Sigma-Aldrich C0150 Cathepsin B from human placenta	Sigma-Aldrich C8571 Cathepsin B from human liver	Sigma-Aldrich SAB5702092 Anti-Cathepsin B Antibody, clone 6I5D7, Rabbit Monoclonal	Sigma-Aldrich C6243 Anti-Cathepsin B antibody, Mouse monoclonal
description lyophilized powder, ≥5 units/mg protein	description buffered aqueous solution, ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)	description -	description clone CB59-4B11, purified from hybridoma cell culture
specific activity ≥5 units/mg protein	specific activity ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)	specific activity -	specific activity -
Gene Information human ... CTSB(1508)	Gene Information human ... CTSB(1508)	Gene Information human ... CTSB(1508)	Gene Information human ... CTSB(1508) rat ... CTSB(64529)
form lyophilized powder	form buffered aqueous solution	form liquid	form buffered aqueous solution
mol wt 24.5 kDa	mol wt -	mol wt 44 kDa	mol wt antigen ~25 kDa
storage temp. −20°C	storage temp. −70°C	storage temp. −20°C	storage temp. −20°C
solubility H₂O: soluble 1.0 mg/mL, clear	solubility -	solubility -	solubility -

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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