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B8131

Sigma-Aldrich

2-(2′-Benzothiazolyl)-5-styryl-3-(4′-phthalhydrazidyl)tetrazolium chloride

90-95%

Synonym(s):

BPST Chloride, BSPT Chloride

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About This Item

Empirical Formula (Hill Notation):
C24H16ClN7O2S
CAS Number:
Molecular Weight:
501.95
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:

Assay

90-95%

storage temp.

2-8°C

SMILES string

[Cl-].O=C1NNC(=O)c2cc(ccc12)-[n+]3nc(\C=C\c4ccccc4)nn3-c5nc6ccccc6s5

InChI

1S/C24H15N7O2S.ClH/c32-22-17-12-11-16(14-18(17)23(33)27-26-22)30-28-21(13-10-15-6-2-1-3-7-15)29-31(30)24-25-19-8-4-5-9-20(19)34-24;/h1-14H,(H,28,29);1H/b13-10+;

InChI key

ZPGCKYFKHCIWHR-RSGUCCNWSA-N

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Use of BSPT in dehydrogenase histochemistry.
W A Shannon
The Histochemical journal, 14(1), 166-168 (1982-01-01)
G Wolf et al.
Neuroscience letters, 161(1), 49-52 (1993-10-14)
The 2-(2'-benzothiazolyl)-5-styryl-3-(4'-phthalhydrazidyl) tetrazolium chloride (BSPT)-tetrazolium salt technique for the electron microscopic demonstration of reduced nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) was used to localize nitric oxide synthase in the normal and excitotoxically lesioned rat hippocampus. The reaction product BSPT-formazan was shown
S Barbey et al.
Pathology, research and practice, 182(6), 805-809 (1987-12-01)
The ultrastructural and histochemical exploration of histiocytes X done by the means of Concanavalin A (Con A), peanut agglutinin (PNA) and BSPT demonstrate that the plasma membrane of histiocytes X shared some properties with all the other cells and is
L Villani et al.
Brain research, 743(1-2), 353-356 (1996-12-16)
The localization of nitric oxide synthase and NADPH-diaphorase was studied in the goldfish retina by means of immunohistochemistry or tetrazolium salt technique. Nitric oxide synthase was found in some small neurons of the inner nuclear layer and in large neurons
Problems encountered with BPST in dehydrogenase histochemistry.
P B Gahan et al.
The Histochemical journal, 13(2), 338-338 (1981-03-01)

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