A8731

Anti-Atg12 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-APG12, Anti-Autophagy related 12 homolog (S. cerevisiae)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~20 kDa
• species reactivity: mouse
• technique(s): immunoprecipitation (IP): 5-10 μL using whole extract of HEK-293T cells co-expressing mouse Atg12 and Atg5-EGFP proteins; western blot: 1:250-1:500 using whole extract of HEK-293T cells expressing mouse Atg12
• UniProt accession no.: Q9CQY1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ATG12(9140) ; mouse ... Atg12(67526)

Description

General description

Atg12 is an autophagy-related protein. It forms a complex with Atg5 and functions in LC3 processing.

The autophagy-related protein 3 (ATG3) gene is mapped to human chromosome 5q22.3. ATG12 is a mammalian homolog of yeast Apg3p/ Atg3 is ubiquitously expressed in human tissues.

Immunogen

synthetic peptide corresponding to amino acids 27-39 of mouse Atg12, conjugated to KLH. The corresponding sequence differs by one amino acid in human.

Application

Anti-Atg12 antibody produced in rabbit has been used for:

• immunoprecipitation
• western blot
• immunohistochemistry staining.

Biochem/physiol Actions

Macroautophagy, usually referred to as autophagy, is a major pathway for bulk degradation of cytoplasmic constituents and organelles. Atg12 is a ubiquitin-like modifier essential for autophagy. Two ubiquitin-like conjugation systems are involved in autophagosome formation: Atg12 and Atg8 conjugation systems. Autophagy is required for normal turnover of cellular components during starvation. It plays an essential role in cellular differentiation, cell death and aging. Defective autophagy may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves