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A7641

Sigma-Aldrich

Albumin from chicken egg white

lyophilized powder

Synonym(s):

Albumin from hen egg white, Ovalbumin

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About This Item

CAS Number:
9006-59-1
EC Number:
232-692-7
MDL number:
MFCD00130424
UNSPSC Code:
12352202
eCl@ss:
42010201
NACRES:
NA.25

biological source

chicken egg white

type

Grade VII

Assay

≥95% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

44287 Da by calculation

purified by

chromatography

technique(s)

bioconjugation: suitable

UniProt accession no.

P01012

storage temp.

2-8°C

Gene Information

chicken ... OVAL(396058)

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General description

Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa.
Ovalbumin, an egg white protein is produced in the oviduct of hen. It has 3 sites of post synthetic modification and an N-terminal acetyl group. This product is free of S-ovalbumin, a heat stable form of egg albumin formed when eggs are stored.

Application

Albumin from chicken egg white has been used:
  • as a component in reaction buffer for in vitro enzymatic assay
  • to simulate dangerous protein toxins
  • as a component in marker solution

The product is useful as a marker (∼45 kDa) for protein electrophoresis. It has been used to produce an animal model of asthma.
Ovalbumin was used as medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil.
It was also used in a study testing intestinal macromolecule absorption in fetal pig after infusion of colostrum in utero.
A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.

Biochem/physiol Actions

Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen.
Ovalbumin is preferred as a nutrient supplement. It can serve as a drug carrier. It possess tumor necrosis releasing factors, that can play a great role in tumor suppression.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1A

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Egg white proteins and their potential use in food processing or as nutraceutical and pharmaceutical agents?A review
Abeyrathne EDNS, et al.
Poultry Science, 92(12), 3292-3299 (2013)
UAF1 is a subunit of multiple deubiquitinating enzyme complexes
Cohn MA, et al.
The Journal of Biological Chemistry, 284(8), 5343-5351 (2009)
Multiplexed immunoassay based on micromotors and microscale tags
Vilela D, et al.
Lab on a chip, 14(18), 3505-3509 (2014)
Intestinal permeability in humans is increased after radiation therapy
Nejdfors P, et al.
Diseases of the Colon and Rectum, 43(11), 1582-1587 (2000)
P T Sangild et al.
Pediatric research, 45(4 Pt 1), 595-602 (1999-04-15)
The ability of the fetal pig intestine to absorb large proteins was investigated in utero. Six pregnant sows were anesthetized (Na pentobarbital) at 99-102 d of gestation (term = 115 +/- 2 d), and a catheter was inserted into the
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Articles

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

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