A7470

Anti-c-Myc Agarose Affinity Gel antibody produced in rabbit

affinity isolated antibody

• Synonym(s): Anti-c-Myc
• NACRES: NA.56
• UNSPSC Code: 12352203
• Conjugate: agarose conjugate
• Clone: polyclonal
• Application: IP, affinity chromatography
• Citations: 274

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: agarose conjugate
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• analyte chemical class(es): proteins (c-Myc)
• technique(s): affinity chromatography: suitable, immunoprecipitation (IP): suitable
• capacity: ≥10 nmol/mL binding capacity (c-Myc tagged fusion protein), ≥7 nmol/mL elution capacity (c-Myc tagged fusion protein)
• UniProt accession no.: P01106
• storage temp.: 2-8°C
• Gene Information: human ... MYC(4609)

Description

General description

The human c-Myc proto-oncogene belongs to MYC oncogene family and is the human cellular homolog of the avian v-Myc gene found in several leukemogenic retroviruses. c-Myc gene is localized on human chromosome 8q24.21. The two major domains of include the N-terminal box regions and the C-terminal basic-region with helix-loop-helix/leucine-zipper motif.

Immunogen

synthetic peptide of the human p62^c-Myc protein.

Application

Anti-c-Myc Agarose Affinity Gel antibody produced in rabbit has been used in:

• chromatin immunoprecipitation (ChIP)
• co-immunoprecipitation
• western blotting
• immunofluorescence
• immunoprecipitation

Biochem/physiol Actions

c-Myc proto-oncogene increased expression is observed in a variety of human tumors. Amplification of Myc gene locus is observed in angiosarcomas and translocation is implicated in Burkitt′s lymphoma. c-Myc is a master regulator and targets a wide variety of metabolic events. An epitope located within amino acid residues 410-419 (EQKLISEEDL) of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tagged fusion proteins. Anti-c-Myc agarose is useful in the purification of expressed c-Myc tagged fusion proteins in bacterial lysates or transfected cells.

Anti-c-Myc recognizes an epitope located on c-Myc tagged fusion proteins.

Physical form

Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Preparation Note

For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze.

Other Notes

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Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable