A6979
Anti-AtMPK4 antibody produced in rabbit
affinity isolated antibody, liquid
Synonym(s):
Anti-Arabidopsis thaliana MPK4
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About This Item
Recommended Products
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
grade
for molecular biology
clone
polyclonal
form
liquid
mol wt
antigen 43 kDa
species reactivity
Arabidopsis thaliana
technique(s)
western blot: 0.5-1 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana and Nicotiana tabacum leaves
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
Arabidopsis thaliana ... ATMPK4(828151)
General description
Anti-AtMPK4 is produced in rabbit using as immunogen a synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK4 (Anti- mitogen-activated protein kinase-4), conjugated to KLH. In Arabidopsis thaliana, 20 genes (MPKs) encode putative plant MAPKs, which can be grouped into four or five classes according to sequence similarity. All plant mitogen-activated protein kinase (MAPKs) are classified into the plant extracellular-regulated kinase (PERK) subfamily.
Mitogen-activated protein kinase-4 (MPK4) is expressed in the nucleus. In pre-mitotic and interphase cells, it is found in the cytoplasm.
Immunogen
synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK4 (amino acids 365-376). This sequence is specific to AtMPK4 (not found in the other 20 known AtMPK isoforms). It shares limited homology (50-60% identity, continuous epitope) to the AtMPK5 and AtMPK12 isoforms.
Application
Anti- mitogen-activated protein kinase-4 (AtMPK4) antibody produced in rabbit has been used for Western blotting.
Anti-AtMPK4 antibody has been used in
- immunoblotting
- immunoprecipitation
- co-immunoprecipitation
- western blotting
Biochem/physiol Actions
In Arabidopsis thaliana putative functions have been assigned to AtMPK3, AtMPK4, and AtMPK6, and all three mitogen-activated protein kinase (MAPKs) have been linked to diverse stress responses, including oxidative stress signaling, pathogen defense, drought, high salt, cold, and touch. Arabidopsis MAPKs, Anti- mitogen-activated protein kinase-3 (AtMPK3), Anti- mitogen-activated protein kinase-4 (AtMPK4), and Anti- mitogen-activated protein kinase-6 (AtMPK6) are rapidly and transiently activated by stress responses, by activation of distinct signal transduction pathways, involving upstream MAPKKKs (e.g. AtANP1 and AtMEKK1) and MAPKKs including AtMKK1/2 and AtMKK4/5.
Mitogen-activated protein kinase-4 (MPK4) negatively modulates systemic-acquired resistance. It functions as a regulator of retrograde signaling for photosynthesis, growth and immune defenses. It also has a role in cytokinesis and microtubule organization.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Mitogen-activated protein kinase 4 is involved in the regulation of mitotic and cytokinetic microtubule transitions in Arabidopsis thaliana
Martina Beck
The New phytologist (2010)
Various abiotic stresses rapidly activate Arabidopsis MAP kinases ATMPK4 and ATMPK6
Ichimura K, et al.
The Plant Journal, 24(5), 655-665 (2000)
MYB75 Phosphorylation by MPK4 Is Required for Light-Induced
Anthocyanin Accumulation in Arabidopsis
Anthocyanin Accumulation in Arabidopsis
Shengnan Li
Plant Cell (2016)
Yingzhong Li et al.
PLoS pathogens, 6(9), e1001111-e1001111 (2010-09-24)
Plant defense responses need to be tightly regulated to prevent auto-immunity, which is detrimental to growth and development. To identify negative regulators of Resistance (R) protein-mediated resistance, we screened for mutants with constitutive defense responses in the npr1-1 background. Map-based
SRFR1 negatively regulates plant NB-LRR resistance protein accumulation to prevent autoimmunity
Li Y, et al.
PLoS Pathogens, 6(9), e1001111-e1001111 (2010)
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