A3076

Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal

clone GE-1, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Human IgE, Monoclonal Anti-Human IgE−Alkaline Phosphatase antibody produced in mouse
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: alkaline phosphatase conjugate
• Clone: GE-1, monoclonal
• Application: DB, ELISA (d)
• Citations: 14

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: secondary antibodies
• clone: GE-1, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): direct ELISA: 1:6,000, dot blot: 1:1,000
• isotype: IgG2b
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Binds human IgE; does not bind human IgG, IgA or IgM.

IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.

Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal anti-human IgE-alkaline phosphatase antibody has been used for :

• immunoblot assays
• direct ELISA
• dot blot

To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.

Biochem/physiol Actions

IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

Analysis Note

The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)