A1293
Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Goat Anti-Mouse IgG (Fab specific)–AP
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About This Item
Recommended Products
biological source
goat
recombinant
expressed in goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse
should not react with
human
technique(s)
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot (chemiluminescent): 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice .
Specificity
Binds all mouse Igs. Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fab fragment. The product does not react with mouse IgG, Fc fragment, or human IgG.
Immunogen
Purified mouse IgG Fab fragment
Application
Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.
Anti-Mouse IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in:
- enzyme-linked immunosorbent assay (ELISA){47
- immunohistochemistry
- western blot
Biochem/physiol Actions
Immunoglobulin G (IgG) helps in opsonization and antibody dependent cell mediated cytotoxicity. It mainly helps in immune defense.
Other Notes
Antibody adsorbed with human IgG.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
Preparation Note
Adsorbed to reduce background staining with human samples.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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