A0157
Ascorbate Oxidase from Cucurbita sp.
lyophilized powder, 1,000-3,000 units/mg protein
Synonym(s):
L-Ascorbate:oxygen oxidoreductase, Ascorbase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-852-6
MDL number:
EC Number:
Specific activity:
1,000-3,000 units/mg protein
Biological source:
plant (Cucurbita spp.)
Pricing and availability is not currently available.
biological source
plant (Cucurbita spp.)
Quality Segment
form
lyophilized powder
specific activity
1,000-3,000 units/mg protein
storage temp.
2-8°C
General description
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.
Application
Ascorbate Oxidase from Cucurbita sp. has been used:
- as a supplement in the culture medium for differentiation into osteoblasts
- to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay
- in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations
Biochem/physiol Actions
Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.
Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.
Physical form
Lyophilized powder containing buffers and sucrose as stabilizer.
Other Notes
One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.
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This Item | |||
|---|---|---|---|
| description lyophilized powder, 1,000-3,000 units/mg protein | description European Pharmacopoeia (EP) Reference Standard | description crystalline, ≥98% | description United States Pharmacopeia (USP) Reference Standard |
| biological source plant (Cucurbita spp.) | biological source synthetic | biological source synthetic | biological source - |
| specific activity 1,000-3,000 units/mg protein | specific activity - | specific activity - | specific activity - |
| form lyophilized powder | form solid | form crystalline | form - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. - |
| Quality Level 200 | Quality Level - | Quality Level 300 | Quality Level - |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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