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Sigma-Aldrich

Phalloidin–Atto 647N

BioReagent, suitable for fluorescence, ≥80% (HPLC)

Synonym(s):

Atto 647N, Atto 647N-Phalloidin

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About This Item

UNSPSC Code:
12171501
NACRES:
NA.32

product line

BioReagent

Assay

≥80% (HPLC)

manufacturer/tradename

ATTO-TEC GmbH

λ

in methanol

UV absorption

λ: 640-646 nm Amax

suitability

suitable for fluorescence

detection method

fluorometric

storage temp.

−20°C

Related Categories

General description

Phalloidin–Atto 647N is a new fluorescentlabel targeting the red spectral region. Atto 647N is a cationic dye, and postcoupling, it carries a net electrical charge of +1.Like most Atto labels, the absorption andfluorescence of Atto 647N are independent of pH between 2-11. Atto 647N issupplied in the form of a mixture containing two isomers having identicalfluorescence and absorption properties. Atto labels have rigid structures thatdo not show any cis-trans-isomerization.

Application

Phalloidin–Atto 647Nis designed to be used for labelling DNA, RNA, or proteins. Fluorescentconjugates of phalloidin are used to label actin filaments for histologicalapplications. Some structural features of phalloidin are required for thebinding to actin.

Features and Benefits

Characteristic features of the Phalloidin Atto488 are:
  1. StrongAbsorption.
  2. HighFluorescence quantum yield.
  3. HighPhotostability.
  4. MinimalTriplet formation.
  5. GoodSolubility.
  6. Excellent Ozone Resistance.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H300 + H310 + H330

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Polarized epithelia develop distinct cell surface domains, with the apical membrane acquiring characteristic morphological features such as microvilli. Cell polarization is driven by polarity determinants including the evolutionarily conserved partitioning-defective (PAR) proteins that are separated into distinct cortical domains. PAR
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