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Key Documents

47753

Sigma-Aldrich

Formate Dehydrogenase from Candida boidinii

liquid (clear), clear brown, 40.0-60.0 U/mL

Synonym(s):

FDH, Formate:NAD+ oxidoreductase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204

biological source

fungus (Candida boidinii)

form

liquid (clear)

mol wt

Mr ~76000

concentration

40.0-60.0 U/mL

color

clear brown

density

1.1 g/mL at 20 °C

storage temp.

−20°C

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Biochem/physiol Actions

Formate dehydrogenase is involved in the stress response of plants and catalyzes the reduction of NAD+ to NADH.

Unit Definition

1 U corresponds to the amount of enzyme which oxidizes 1 μmol sodium formate (Cat. No. 71539) per minute at pH 7.6 and 25 °C

Other Notes

Preferred enzyme for regenerating NADH from NAD

comparable product

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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K. Drauz et al.
Enzyme Catalysis in Organic Synthesis, 597-597 (1995)
Yuan Lu et al.
Applied biochemistry and biotechnology, 167(4), 732-742 (2012-05-18)
To improve the hydrogen productivity and examine the hydrogen evolution mechanism of Clostridium paraputrificum, roles of formate in hydrogen evolution and effects of introducing formate-originated NADH regeneration were explored. The formate-decomposing pathway for hydrogen production was verified to exist in
Yutaka Amao et al.
Faraday discussions, 155, 289-296 (2012-04-05)
Solar fuels, such as hydrogen gas produced from water and methanol produced from carbon dioxide reduction by artificial photosynthesis, have received considerable attention. In natural leaves the photosynthetic proteins are well-organized in the thylakoid membrane. To develop an artificial leaf
Mark Pryjma et al.
Journal of bacteriology, 194(15), 3803-3813 (2012-05-29)
Campylobacter jejuni is a food-borne bacterial pathogen that colonizes the intestinal tract and causes severe gastroenteritis. Interaction with host epithelial cells is thought to enhance severity of disease, and the ability of C. jejuni to modulate its metabolism in different
Frances L Shaw et al.
Journal of bacteriology, 194(15), 3814-3823 (2012-05-23)
The food-borne bacterial pathogen Campylobacter jejuni efficiently utilizes organic acids such as lactate and formate for energy production. Formate is rapidly metabolized via the activity of the multisubunit formate dehydrogenase (FDH) enzyme, of which the FdhA subunit is predicted to

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