41367

Anti-Rabbit IgG−Abberior^® STAR 580 antibody produced in goat

• Synonym(s): Abberior^® STAR 580-Anti-Rabbit IgG antibody produced in goat
• NACRES: NA.45
• UNSPSC Code: 12352200

Properties

• biological source: goat
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: rabbit
• concentration: ~1 mg/mL
• fluorescence: λ_ex 587 nm; λ_em 607 nm in PBS, pH 7.4
• storage temp.: −20°C

Description

General description

Abberior STAR 580 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 580 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with excitation wavelength between 550 - 600 nm. Abberior STAR 580 can substitute dyes like Cy3^™, Atto^™ 590 or Alexa Fluor^™ 568. For STED microscopy, Abberior STAR 580 can be used most efficiently with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 580 is the ideal partner for Abberior STAR RED or Abberior STAR 635P to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 587 (PBS pH 7.4), 584 (H₂O), 584 (MeOH)
Extinction Coefficient, ε_max [M^-1cm^-1]: 85 000 (PBS pH 7.4), 85 000 (H₂O), 95 000 (MeOH)
Correction Factor, CF₂₆₀ = ε₂₆₀/ε_max: 0,17 (PBS pH 7.4)
Correction Factor, CF₂₈₀ = ε₂₈₀/ε_max: 0,17 (PBS pH 7.4)
Fluorescence Maximum, λ_em [nm]: 607 (PBS pH 7.4), 607 (H₂O), 604 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,5 (PBS pH 7.4)

Application

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior^®

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

Cy3 is a trademark of Cytiva

abberior is a registered trademark of Abberior GmbH

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable