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Atto 655-Streptavidin

Name: Atto 655-Streptavidin
Brand: SIGMA

BioReagent, suitable for fluorescence

Synonym(s):

Streptavidin-Atto 655

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352116

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Properties

product line

BioReagent

Quality Segment

100

form

powder

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

fluorescence

λ_ex 655 nm; λ_em 680 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence, suitable for gel electrophoresis

storage temp.

−20°C

Description

Application

Atto 655 is a fluorescent label with high molecular absorption (125.000) and quantum yield (0.30), as well as sufficient stoke′s shift (excitation maximum 663 nm, emission maximum 684 nm). There is very little cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Atto 655 has been coupled to streptavidin to enable various types of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays.

Analysis Note

free of unconjugated dye

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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This Item	09260	06966	RSTREP-RO
Sigma-Aldrich 02744 Atto 655-Streptavidin	Sigma-Aldrich 09260 Atto 425-Streptavidin	Sigma-Aldrich 06966 Atto 655-Biotin	Roche RSTREP-RO Streptavidin, recombinant
suitability suitable for fluorescence, suitable for gel electrophoresis	suitability -	suitability suitable for fluorescence	suitability suitable for immunological tests
fluorescence λ_ex 655 nm; λ_em 680 nm in 0.1 M phosphate pH 7.0	fluorescence λ_ex 437 nm; λ_em 477 nm in 0.1 M phosphate pH 7.0	fluorescence λ_ex 655 nm; λ_em 680 nm in 0.1 M phosphate pH 7.0	fluorescence -
solubility DMF: soluble, acetonitrile: soluble, DMSO: soluble	solubility H₂O: soluble	solubility DMF: soluble, DMSO: soluble, acetonitrile: soluble	solubility water: 10 mg/L (mA₄₀₅=100)
form powder	form powder	form powder	form liquid
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. -10 to -25°C
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level -

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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