G6257

Glutaraldehyde solution

Grade II, 25% in H₂O

• Synonym(s): Glutaric dialdehyde solution, Pentane-1,5-dial
• Linear Formula: OHC(CH₂)₃CHO
• CAS Number: 111-30-8
• Molecular Weight: 100.12
• Beilstein: 605390
• MDL number: MFCD00007025
• UNSPSC Code: 12352114
• PubChem Substance ID: 24895250
• NACRES: NA.21

Properties

• type: Grade II
• Quality Level: 200
• form: liquid
• concentration: 25% in H₂O
• technique(s): electron microscopy: suitable
• color: colorless
• useful pH range: 2.9
• mp: -10 °C ((14 °F ))
• solubility: water: soluble
• application(s): cell analysis; sample preparation
• SMILES string: [H]C(CCCC([H])=O)=O
• InChI: 1S/C5H8O2/c6-4-2-1-3-5-7/h4-5H,1-3H2
• InChI key: SXRSQZLOMIGNAQ-UHFFFAOYSA-N

Description

General description

Glutaraldehyde, known as Glutaric dialdehyde or Pentane-1,5-dial, serves as a versatile reagent with applications spanning various research domains, including cell biology and biochemical research. It finds applications as a protein crosslinker, aids in enzyme immobilization for microscopy, and contributes to histochemistry and cytochemistry processes. As an amine-reactive homobifunctional crosslinker, it acts as a cell fixative before procedures like SDS-PAGE, staining, or electron microscopy, making it a valuable tool for diverse morphological studies.

Glutaraldehyde demonstrates efficacy in crosslinking amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, it allows direct coupling of biotin hydrazides to nucleic acids, offering potential applications in conjugating fluorescent hydrazides and hydroxylamines to DNA. Moreover, in histology studies, Glutaraldehyde is employed for preserving tissue sections and preparing them for detailed examination.

Application

Glutaraldehyde solution has been used:

• for fixation of mouse[1] and esophageal cancer cell lines[2]
• to crosslink amyloid-β protein[3]
• to crosslink gelatin in 3D scaffold preparation of trachea[4]

Biochem/physiol Actions

Glutaraldehyde is an effective protein crosslinker and finds application in techniques like enzyme immobilisation microscopy, histochemistry and cytochemistry. It exists in different forms under acidic or neutral conditions.[5] It is toxic to aquatic organisms.[6] Its allergic nature leads to hypersensitivity reactions. Contact of glutaraldehyde vapors in endoscopy contributes to Colitis.[7]

Features and Benefits

• Versatile and adaptable for wide variety of research applications
• Ready-made solution reduces the need for preparation time

Packaging

Not refrigerated en route

Caution

This grade is not recommended for use as an electron microscopy fixative.

Preparation Note

Not specially purified.

Other Notes

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To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Disclaimer

“The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.”

Safety Information

• Pictograms: GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H314,H317,H334,H335,H410
• Precautionary Statements: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Supplementary Hazards: EUH071
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves