PC709
Anti-MYH (33-51) Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-MutY Homolog
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, rat
Application:
—
Citations:
-
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:1500)
Immunofluorescence (1:500)
Paraffin Sections )
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MUTYH(4595)
General description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~52-57 kDa isoforms of MYH.
Recognizes the MYH protein and several of its approximately 10 isoforms in PC12, Jurkat, and HeLa cells.
This Anti-MYH (33-51) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Paraffin Sections for the detection of MYH (33-51).
Immunogen
Human
a synthetic peptide (QEGRQKHAKNNSQAKPSAC) corresponding to amino acids 33-51 of human MYH
Application
Immunoblotting (1:1500)
Immunofluorescence (1:500)
Paraffin Sections (see application references)
Immunofluorescence (1:500)
Paraffin Sections (see application references)
Physical form
Undiluted serum.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
PC12, Jurkat, or HeLa cells
PC12, Jurkat, or HeLa cells
Other Notes
Detects MYH and several of its approximately ten isoforms. In Jurkat cells the ~52 kDa and ~53 kDa variants were found in the nuclear fraction while the ~57 kDa protein is found in the mitochondria. Antibody should be titrated for optimal results in individual systems.
Wang, G., et al. 2000. Nucleic Acids Res.28, 2135.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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