MABC83

Anti-Vps4/SKD1 Antibody, clone 5E6.1

clone 5E6.1, from mouse

• Synonym(s): Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5E6.1, monoclonal
• species reactivity: mouse, human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_004860
• UniProt accession no.: O75351
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... VPS4B(9525)

Description

General description

Vacuolar protein sorting-associated protein 4B (Vps4), or SKD1, is a ubiquitous AAA ATPase involved in endosomal vesicle trafficking. Trafficking of vesicles containing proteins such as receptors, from the endosome to the cytoplasm, is an important regulatory mechanism for cell-surface receptors and the signaling pathways that involve those receptors. Such endosomal vesicles are shuttled from the endosome to the cytoplasm by multivesicular bodies (MVB). MVBs are large multi-protein complexes that assemble and disassemble during this process. Previous studies have indicated that the yeast homologue of Vps4, Vps4p (60 % sequence identity to Vps4), interacts with endosomes via its N-terminal microtubule interacting and trafficking (MIT) domain, and also associates with a number of MVB proteins including Vps2p, Bro1p, Vps20p, Vta1p, and Did2p/Chm1p. Although the precise role of Vps4 is still unclear, previous studies have suggested that it interacts with the ESCRT-III complex involved in the sorting of MVBs and has been associated with virus-loaded vesicles such those in HIV-infected cells.

Immunogen

Linear peptide corresponding to human Vps4/SKD1.

Application

Anti-Vps4/SKD1 Antibody, clone 5E6.1 is an antibody against Vsp4/SKD1 for use in Western Blotting, ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Vps4/SKD1 in NIH/3T3, A431, and HeLa cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Western Blot in human skeletal muscle tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Vps4/SKD1 in 10 µg of human skeletal muscle tissue lysate.

Target description

~52 kDa observed. The calculated molecular weight of this protein is ~50 kDa Uniprot describes that this protein is autophosphoralated upon DNA damage.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human skeletal muscle tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable