CC054

Human Collagen Type III

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

• Synonym(s): Collagen III from human placenta, alpha 1 Type III Collagen
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.75
• Biological source: human
• Form: solution
• Technique(s): cell culture | mammalian: suitable
• Concentration: 1 mg/mL
• Assay: 90% (Human collagen type III, SDS-PAGE)

Properties

• Product Name: Human Collagen Type III, 1 mg/mL, solution, Bioreagent, suitable for cell culture
• biological source: human
• Quality Segment: 200
• assay: 90% (Human collagen type III, SDS-PAGE)
• form: solution
• manufacturer/tradename: Chemicon^®
• concentration: 1 mg/mL
• technique(s): cell culture | mammalian: suitable
• impurities: <1% Human collagen types II, IV-VI, and non-collagen proteins, <10% Human collagen type I
• input: sample type epithelial cells; sample type mesenchymal stem cell(s); sample type: human embryonic stem cell(s); sample type induced pluripotent stem cell(s); sample type neural stem cell(s); sample type hematopoietic stem cell(s); sample type pancreatic stem cell(s)
• solubility: water: soluble at 20 °C
• NCBI accession no.: NM_000090.3
• UniProt accession no.: P02461
• binding specificity: Peptide Source: Fibrinogen
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... COL3A1(1281)

Description

General description

The COL3A1 gene mapped to human chromosome 2q32.2, encodes the alpha 1 chain of type III collagen. Type III collagen is an extracellular matrix protein containing a long triple-helical domain made of three alpha 1 chains. Collagen III is a major structural component in hollow organs such as large blood vessels, the uterus and the bowel. Human type III collagen is purified by serial salt precipitations, alcohol precipitation, and DEAE chromatography of an acetic acid extraction of human placenta, following mild pepsin digestion. Product Source: Human placenta, negative for HbsAG, HCV and HIV 1 & 2 antibodies.

Application

Human Collagen Type III has been used:

• as an extracellular matrix (ECM) protein in microarray analysis, to coat nitrocellulose film slides for probing and detection experiments[1]
• to coat microfluidic migration devices and to study the impact of extracellular matrix (ECM) on cell migration
• as a positive control in Gpr56 PRESTO-Tango assay and GTP-Rho pull-down assay, to evaluate its effects on cellular signaling and interactions[2]

Biochem/physiol Actions

Collagen III is initially produced as procollagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the procollagen molecule is modified by the cell. The type III procollagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen tissue strengthening function

Physical form

Liquid in 0.5 M acetic acid, pH 2.5.

Preparation Note

Maintain at -20ºC in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Retention of native structure was confirmed by ability to form microfibrils.

Other Notes

Composition: [a1(III)]3, native triple helix. Ability to form native helical structure verified by ORD measurement, competence in microfibril formation and reactivity with anti-collagen type-specific monoclonal antibodies

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable