Goat IgG (H+L) (absorbed for minimal cross-reactivity to Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins)
Application
ELISA and Western Blotting at 1:5000 - 1:50,000 dilution.Optimal working dilutions must be determined by the end user.
Research Category Secondary & Control Antibodies
Research Sub Category Secondary Antibodies Adsorbed for Dual Labeling
This Donkey anti-Goat IgG Antibody, Alkaline Phosphatase conjugate, Species Adsorbed is validated for use in ELISA, WB for the detection of Donkey Goat IgG.
Physical form
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 8.0.
RECONSTITUTION:
Reconstitute to 1.0 mg/mL with 0.5 mL sterile distilled water.
Storage and Stability
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After further dilution do not use for more than 1 day. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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