AB2999

Anti-BNIP3 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_004043
• UniProt accession no.: Q12983
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... BNIP3(664)

Description

General description

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) is a member of the NIP3 family, a singular group of death-inducing, mitochondrial proteins. BNIP3 is comprised of several domains of interest; a highly conserved domain (CD), a C-terminal transmembrane (TM) domain, and a BH3 domain. Strongly upregulated by hypoxia, BNIP3 induces a caspase-independent cell death characterized by mitochondrial damage. Overexpression has been observed in the brain after stroke and in the adult heart following myocardial infarction. Recent studies have shown BNIP3 to be involved in cell growth inhibition through suppression of the mTOR pathway and that, when localized to the nucleus, it has the paradoxical capability of repressing apoptosis in tumors by binding the AIF expression gene.

Specificity

Other homologies: Rat, equine, and ox (90% sequence homology).

Immunogen

Epitope: Internal (C-terminal half).

KLH-conjugated linear peptide corresponding to an internal sequence within the C-terminal half of human BNIP3.

Application

Anti-BNIP3 Antibody detects level of BNIP3 & has been validated for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected hypoxia-induced BNIP3 upregulation in human glioblastoma-astrocytoma, epithelial-like U87 cells (Courtesy of Spencer Gibson, Manitoba Institute of Cell Biology).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in PC3 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected BNIP3 in 10 µg of PC3 cell lysate.

Target description

~60 kDa (dimer) observed. BNIP3 may be observed as a 30 kDa monomer and/or a 60 kDa dimer.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
PC3 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable