Purified mouse monoclonal antibody. Recognizes the Lewis x type 2 oligosaccharide chain.
Recognizes the Lewis x carbohydrate chain in normal kidney.
This Anti-Lewis x Mouse mAb (P12) is validated for use in Paraffin Sections for the detection of Lewis x.
Immunogen
Human
fresh (non-cultured) human placenta cells
Application
Paraffin Sections (1:10-1:120)
Warning
Toxicity: Standard Handling (A)
Physical form
Tissue Culture Supernatant
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control Normal kidney tissue
Other Notes
For paraffin sections, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. Variables associated with assay conditions will dictate the proper working dilutions.
Ree, H.J., et al. 1991. Cancer67, 1338. Cordon-Cardo, C., et al. 1986. Int. J. Cancer37, 667. Sakamoto, J., et al. 1986. Cancer Res. 46, 1553 Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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