234138

Human Collagen Type I

from human skin, powder

• Synonym(s): Collagen, Type I, Human Skin
• CAS Number: 9007-34-5
• MDL number: MFCD00003075
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Collagen, Type I, Human Skin,
• biological source: human skin
• Quality Level: 100
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• dilution: (compatible for the preparation of collagen gels)
• impurities: ≤0.1% subdermal hair
• solubility: acetic acid: 5 mg/mL (0.5M)
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Collagen type 1 is a fibrillar collagen present abundantly in skin, bone, vasculature, and calcified tissues of teeth and bone. It is also seen in organs like the heart and lungs.[1] Collagen type 1 is a heterotrimer consisting of two α1 (CIα1) and one α2 (CIα2) chains with a helical region made of repeating units of proline and hydroxyproline.[2]

Application

Collagen Type I, Human Skin has been used as a skin protein to study the effect of age-related NADH oxidase (ar-NOX)-catalyzed oxidation on skin proteins.[3] It has also been used as a control sample in High-resolution mass spectrometry (HRMS) analysis.[2]

Biochem/physiol Actions

Collagen type 1 is a main component of the dermal extracellular matrix (ECM) and participates in skin elasticity.[4] Mutations in collagen type 1 are associated with osteogenesis imperfecta and Ehlers–Danlos syndrome.[5]

Warning

Toxicity: Standard Handling (A)

Physical form

Salt-free.

Preparation Note

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

The recommended concentration is 0.01 to 0.5 M Acetic Acid (or another acidic buffer), with a maximum concentration of 5 mg/ml. The product has been tested at 0.5 M Acetic Acid and is stable for 1 month at 2-8°C. After reconstitution, aliquot and freeze at -20°C. Stock solutions remain stable for up to 6 months at this temperature.

Other Notes

Gallop, P.M., and Siefert, S. 1963. Methods Enzymol. 6, 635.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable