1.25079

Fractogel^® EMD DEAE (M)

MiniChrom Fractogel^® DEAE (M), volume 5 mL, prepacked column

• Synonym(s): MiniChrom Column Fractogel^® DEAE (M), 5 ml
• UNSPSC Code: 41115711

Properties

• ligand: diethyl-(2-hydroxypropyl)aminoethyl
• Quality Level: 100
• material: polypropylene housing
• product line: Fractogel^®
• form: prepacked column
• parameter: ≤8 bar pressure; ~170 cm/hr max. flow rate
• bed L: 100 mm
• bed volume: 5 mL
• column I.D.: 8 mm
• volume: 5 mL
• matrix active group: Methacrylate
• mean particle size: 40-90 μm
• capacity: ~100 mg, BSA binding capacity
• application(s): gene therapy; mAb; vaccine development
• separation technique: anion exchange
• storage temp.: 15-25°C

Description

General description

Weak cation exchange Fractogel^® EMD DEAE (M) resin in a convenient pre-packed 5 ml column

Features and Benefits

MiniChrom Column Fractogel^® DEAE (M), 5 ml enables:

• Excellent binding to large viruses and plasmid DNA
• Homogenous binding with high selectivity and purity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany