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1.07518

Sigma-Aldrich

Pyronine G (C.I. 45005)

for microscopy Certistain®

Synonym(s):

Pyronine G (C.I. 45005), (C.I. 45005) for microscopy Certistain®

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About This Item

Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
92-32-0
Molecular Weight:
302.80
MDL number:
MFCD00011725
UNSPSC Code:
41116130
EC Index Number:
202-147-8

form

crystals

composition

Dye, ≥75% spectrophotometric assay

mp

250-260 °C

εmax

2340-3160 at 546-551 nm in 50% ethanol at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

General description

Pyronine G (C.I. 45005) is a Certistain dry dye for DNA and RNA staining in methyl green-pyronine G staining in histological sections. It is also described as a dye for staining RNA and DNA.
As CERTISTAIN dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.

Analysis Note

Dye content (spectrophotometrically): ≥ 75 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 546 - 551 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 2340 - 3160
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Shigeo Ohba et al.
Cell reports, 31(2), 107518-107518 (2020-04-16)
The metabolic enzyme phosphoglycerate mutase 1 (PGAM1) is overexpressed in several types of cancer, suggesting an additional function beyond its established role in the glycolytic pathway. We here report that PGAM1 is overexpressed in gliomas where it increases the efficiency

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