1.00950

Chromocult^® Enterococci-Agar

Selective culture medium for the isolation and differentiation of Enterococci

• Synonym(s): Enterococcus Agar, Chromogenic Enterococci agar
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Quality Level: 200
• form: medium granules (dehydrated (DCM))
• manufacturer/tradename: GranuCult^®
• technique(s): microbiological culture: suitable
• pH: 6.8-7.2 (25 °C, 33 g/L in H₂O)
• solubility: 33 g/L
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Enterococcus spp.

Description

General description

The presence of Enterococci, especially E. faecalis, E. faecium, E. durans and E. hirae, serves as an indicator for fecal contamination. The Enterococci-Agar medium is provided with a unique chromogenic substrate that is acted on by various Enterococci species to produce red colonies that can easily be identified. Non-Enterococci species produce blue/violet, turquoise or even colorless colonies which can easily be distinguished from the red. The medium is also provided with sodium azide and ox bile which inhibits the growth of any accompanying microbial flora.

Application

Chromocult^® Enterococci-Agar can be used to isolate, differentiate and enumerate Enterococci present in various foodstuff, water or animal feed. It can also be used as for determination of fecal contamination in foodstuff, water or animal feed

Analysis Note

Appearance (clearness): clear
Appearance (color): yellowish
pH-value (25 °C): 6.8 - 7.2
Recovery on test medium (Enterococcus faecium ATCC 882): ≥ 50 %
Recovery on test medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 70 %
Recovery on test medium (Enterococcus durans ATCC 6056): ≥ 70 %
Recovery on test medium (Enterococcus hirae ATCC 8043 (WDCM 00089)): ≥ 70 %
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≤ 0.01 %
Recovery on test medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 0.01 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≤ 0.01 %
Colony color (Enterococcus faecium ATCC 882): red
Colony color (Enterococcus faecalis ATCC 19433 (WDCM 00009)): red
Colony color (Enterococcus durans ATCC 6056): red
Colony color (Enterococcus hirae ATCC 8043 (WDCM 00089)): red
Colony color (Aerococcus viridans ATCC 10400): pink-red to violet
Growth (Aerococcus viridans ATCC 10400): inhibited
Incubation: 24 hrs.; 35 °C; aerobic.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3