SAP30 (Sin3A-associated protein) is a widely expressed nuclear protein that is functionally conserved between human and yeast. It is a component of the multiprotein histone deacetylase complex containing the transcriptional repressor Sin3, multiple histone deacetylases (HDAC1 and HDAC2), histone binding proteins (RbAp46 and RbAp48) and other polypeptides. SAP30 can recruit the deacetylase complex to promoter DNA in a Sin3 independent manner and is involved in the recruitment of the mSin3 complex to a specific subset of N-CoR corepressor complexes. The latter function suggests a role for SAP30 in N-CoR-mediated repression. Furthermore, SAP30 may be involved in imparting target specificity to the deacetylase complex.
Specificity
Not tested in other species.
Recognizes yeast SAP30 at ~27 kDa.
Immunogen
Epitope: a.a. 141-157
Immunogen was a synthetic peptide corresponding to a.a. 141-157 of yeast SAP30, conjugated to KLH.
Application
Anti-SAP30 (yeast) Antibody is a Rabbit Polyclonal Antibody for detection of SAP30 (yeast) also known as Sin3 corepressor complex subunit SAP30 & has been validated in WB.
Research Category Epigenetics & Nuclear Function
Research Sub Category Histones
Chromatin Biology
Quality
Western Blot Analysis: 1 μg/ml of this antibody detected SAP30 in a yeast extract.
Target description
~27 kDa
Physical form
Format: Purified
Protein A purified IgG in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% NaN3.
Storage and Stability
Stable for 1 year at 2-8°C from date of shipment.
Analysis Note
Control Yeast extract
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
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Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Recently, histone acetylation and deacetylation were found to be catalyzed by structurally distinct, multisubunit complexes that mediate, respectively, activation and repression of transcription. Here, we identify SAP30
The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30
Specific initiation by RNA polymerase I in a whole-cell extract from yeast.
Schultz, M C, et al.
Proceedings of the National Academy of Sciences of the USA, 88, 1004-1008 (1991)
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