05-386
Anti-Vinculin Antibody, clone V284
clone V284, Upstate®, from mouse
Synonym(s):
Anti-CMD1W, Anti-CMH15, Anti-HEL114, Anti-MV, Anti-MVCL
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
V284, monoclonal
species reactivity
human, rat, mouse, rabbit, chicken
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... VCL(7414)
Specificity
Vinculin
Immunogen
Human Vinculin
Application
Anti-Vinculin Antibody, clone V284 detects level of Vinculin & has been published & validated for use in IP, WB, IH.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Quality
routinely evaluated by immunoblot on RIPA lysates of human A431 cells, lysates from chicken embryonic fibroblasts (CEF), or mouse 3T3/A31 fibroblasts
Target description
117kDa
Physical form
Format: Purified
PBS, pH 7.4, containing 0.05% sodium azide and 1mg/ml BSA
Protein A chromatography
Storage and Stability
2 years at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Paul G Leonard et al.
Nature chemical biology, 12(12), 1053-1058 (2016-10-25)
Despite being crucial for energy generation in most forms of life, few if any microbial antibiotics specifically inhibit glycolysis. To develop a specific inhibitor of the glycolytic enzyme enolase 2 (ENO2) for the treatment of cancers with deletion of ENO1
Unique morphology and focal adhesion development of valvular endothelial cells in static and fluid flow environments.
Butcher, JT; Penrod, AM; Garcia, AJ; Nerem, RM
Arteriosclerosis, Thrombosis, and Vascular Biology null
X Wu et al.
The Journal of biological chemistry, 276(32), 30285-30292 (2001-05-31)
The L-type calcium channel is the major calcium influx pathway in vascular smooth muscle and is regulated by integrin ligands, suggesting an important link between extracellular matrix and vascular tone regulation in tissue injury and remodeling. We examined the role
Zeinab Chitforoushzadeh et al.
Science signaling, 9(431), ra59-ra59 (2016-06-09)
Signal transduction networks coordinate transcriptional programs activated by diverse extracellular stimuli, such as growth factors and cytokines. Cells receive multiple stimuli simultaneously, and mapping how activation of the integrated signaling network affects gene expression is a challenge. We stimulated colon
Millie Shah et al.
Molecular & cellular proteomics : MCP, 16(4 suppl 1), S244-S262 (2017-02-09)
Cellular responses to stimuli involve dynamic and localized changes in protein kinases and phosphatases. Here, we report a generalized functional assay for high-throughput profiling of multiple protein phosphatases with subcellular resolution and apply it to analyze coxsackievirus B3 (CVB3) infection
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