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814471

TOYOPEARL® AF-Tresyl-650M Bulk Media

phase tresyl-activated, bottle of 5 g, 65 μm particle size

Synonym(s):

TOYOPEARL® AF-Tresyl-650M Bulk Media

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About This Item

UNSPSC Code:
41115700

description

AF-Tresyl-650M

product line

TOYOPEARL®

form

powder

packaging

bottle of 5 g

manufacturer/tradename

Tosoh TOYOPEARL® AF, 14471

extent of labeling

80 μmol/g dry resin ligand density

parameter

3 bar max. pressure

technique(s)

HPLC: suitable

matrix

hydroxylated methacrylic polymer particle platform

matrix active group

tresyl-activated phase

particle size

65 μm

pore size

1000 Å

separation technique

affinity

storage temp.

2-8°C

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General description

Toyopearl AF-Tresyl-650 resin is an activated resin which readily binds to amine and thiol groups. The resin is provided dry for introduction into a buffered solution containing protein or other ligand in neutral to slightly alkaline (pH 7-8) conditions.

Application

TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.

Physical form

Shipped in dry, free flowing powder.

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Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

related product

Product No.
Description
Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Precautionary Statements

P261 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
026H0207
026H0208

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S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;

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