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STRN250

Sigma-Aldrich

Spectrum™ Plant Total RNA Kit

greener alternative

sufficient for 250 purifications

Synonym(s):

Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

usage

sufficient for 250 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

RNA purification: suitable

greener alternative category

Aligned,

storage temp.

15-25°C

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General description

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Application

Spectrum®Plant Total RNA Kit has been used:
  • to extract RNA from the material ground in liquid nitrogen
  • to isolate total RNA from mutant and wt (wild type) plantlets
  • to extract total RNA from leaf samples
  • to extract RNA from all plant tissues

Features and Benefits

  • Yields up to 60 μg of pure concentrated RNA per prep
  • Efficient protocol allows for RNA purification in 30 min or less
  • Specially designed for research with difficult plant tissues

Legal Information

Specac is a registered trademark of Specac Ltd.
Spectrum is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Target Organs

Liver,Heart

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Certificates of Analysis (COA)

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Anantha Peramuna et al.
Biochemical and biophysical research communications, 500(2), 418-422 (2018-04-17)
Securing a molecular toolbox including diverse promoters is essential for genome engineering. However, native promoters have limitations such as the available number or the length of the promoter. In this work, three short synthetic promoters were characterized by using the
Iodine and Selenium Biofortification with Additional Application of Salicylic Acid Affects Yield, Selected Molecular Parameters and Chemical Composition of Lettuce Plants (Lactuca sativa L. var. capitata).
Smolen S
Frontiers in Plant Science, 7, 1553-1553 (2016)
Martin-Timothy O'Donoghue et al.
Journal of experimental botany, 64(12), 3567-3581 (2013-07-28)
Bryophytes, the most basal of the extant land plants, diverged at least 450 million years ago. A major feature of these plants is the biphasic alternation of generations between a dominant haploid gametophyte and a minor diploid sporophyte phase. These
Transcriptomic Analysis of Drought-Sensitive and Tolerant Genotypes of Perennial Ryegrass (Lolium perenne L.)
Breeding Grasses and Protein Crops in the Era of Genomics, 79(6), 160-165 (2018)
Prasanta K Dash et al.
GM crops & food, 5(2), 106-119 (2014-07-30)
A robust phenotypic plasticity to ward off adverse environmental conditions determines performance and productivity in crop plants. Flax (linseed), is an important cash crop produced for natural textile fiber (linen) or oilseed with many health promoting products. This crop is
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Related Content

Spectrum™ Plant Total RNA Kit

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

DNA & RNA Purification

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DNA & RNA Purification

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

DNA & RNA Purification

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

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