The kit is optimized for use in determination of acid phosphatase in biological samples (e.g. plasma, serum, cell lysate, tissue samples.) This kit has not been tested for use with cell culture supernatant. The presence of phenol red may interfere with results. In addition, the cultured cell line may not secrete sufficient AP to be detected. It would be up to the end user to determine suitability.
MAK446
Acid Phosphatase Assay Kit
Sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
Recommended Products
Quality Level
input
serum
plasma
tissue sample(s)
cell lysate
detection method
colorimetric
relevant disease(s)
cancer, prostate tumor; gastrointestinal diseases
storage temp.
−20°C
General description
Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.
Application
The Acid Phosphatase Assay Kit may be used for:
- Cancer Research
- Gastrointestinal Research
- Metabolism Research
Features and Benefits
Efficient and Precise: Requires only 20 μL of sample with a 30-minute assay reaction to detect acid phosphatase levels across a broad range from 0.05 to 60 U/L, providing both convenience and sensitivity.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Suitability
The kit is suitable for the determination of acid phosphatase in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)
Principle
The non-radioactive, colorimetric Acid Phosphatase Assay Kit is based on the cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at A405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
Target Organs
Nervous system,Kidney,Liver,Skin
Storage Class Code
8A - Combustible corrosive hazardous materials
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Hi, Im trying to measure the released acid phosphatase activity by using cell culture medium. Can I make that experiment with this kit or it is just for the cell lysate etc. ? Thank you for your answer already!
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