A9460

Annexin V from human placenta

≥90% (SDS-PAGE), buffered aqueous solution

• Synonym(s): Annexin V, Calphosbindin I, Lipocortin V, PAP-1
• UNSPSC Code: 41106305
• NACRES: NA.32

Properties

• biological source: human placenta
• Quality Level: 200
• Assay: ≥90% (SDS-PAGE)
• form: buffered aqueous solution
• mol wt: 33 kDa (four subunits)
• technique(s): cell based assay: suitable
• impurities: Human Source(HCV), tested negative
• solubility: water: soluble
• suitability: suitable for molecular biology
• UniProt accession no.: P08758
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... ANXA5(308)

Description

General description

Research area: Apoptosis

Annexin V is a calcium-dependent phospholipid-binding protein. It is expressed in high concentration in cells with barrier functions, such as vascular endothelium, epithelial cells in bile ducts, placental trophoblasts, mammary ducts, renal tubules, and nasal epithelium.

Application

Annexin V from human placenta has been used:

• to verify the involvement of phosphatidylserine in adhesion using flow cytometry
• to test adhesion specificity of phosphatidylserine
• for synthesis of NIR700-Annexin-V imaging probe
• in dynamic light scattering (DLS) assay to test the effects of various treatments on virion aggregation

Biochem/physiol Actions

Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. The cellular changes involved in the apoptotic process include loss of phospholipid asymmetry during the early stages. This phenomenon is universal and is not limited to stimulus or to mammalian cells, but also occurs in insect and plant cells. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg²⁺ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidylserine becomes translocated to the external surface of the cell membrane. Since the movement of phosphatidylserine from the internal membrane surface to the external surface is an early indicator of apoptosis, annexin V and its conjugates that interact strongly and specifically with phosphatidylserine may be used to detect apoptosis. Annexin V conjugates can be used to detect apoptotic cells significantly earlier than DNA-based assays. Fluorescent dye labeled annexin V have applications in flow cytometry, fluorescence microscopy, and laser scanning cytometry.

It is a widely used marker for identifying apoptotic cells due to its high affinity for phosphatidylserine, which is normally located on the inner leaflets of membranes but moves to the cell surface during programmed cell death.

Packaging

Package size based on protein content

Physical form

Solution in 40 mM Tris-HCl, pH 7.5, containing 150 mM NaCl, 1 mM DTT and 0.05% sodium azide.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable