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93834

Sigma-Aldrich

2-Chloro-4-nitrophenyl-α-D-maltotrioside

chromogenic, ≥95% (HPLC), powder or crystals

Synonym(s):

2-chloro-4-nitrophenyl O-α-D-glucopyranosyl-(1→4)-O-α-D-glucopyranosyl-(1→4)-α-D-glucopyranoside, CNP-G3

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About This Item

Empirical Formula (Hill Notation):
C24H34ClNO18
CAS Number:
118291-90-0
Molecular Weight:
659.98
MDL number:
MFCD11045274
UNSPSC Code:
12352204
PubChem Substance ID:
329770276
NACRES:
NA.32

Product Name

2-Chloro-4-nitrophenyl-α-D-maltotrioside, ≥95% (HPLC)

Assay

≥95% (HPLC)

form

powder or crystals

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O[C@@H]2CO)O[C@H]3[C@H](O)[C@@H](O)[C@H](O[C@@H]3CO)Oc4ccc(cc4Cl)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C24H34ClNO18/c25-8-3-7(26(37)38)1-2-9(8)39-22-18(35)15(32)20(11(5-28)41-22)44-24-19(36)16(33)21(12(6-29)42-24)43-23-17(34)14(31)13(30)10(4-27)40-23/h1-3,10-24,27-36H,4-6H2/t10-,11-,12-,13-,14+,15-,16-,17-,18-,19-,20-,21-,22+,23-,24-/m1/s1

InChI key

KMYYNUOXSFGLNX-XFNLHOCBSA-N

Application

2-Chloro-4-nitrophenyl-α-D-maltotrioside has been used as a substrate in assays to determine the enzymatic activity of α-amylase.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL7649
BCCL1464
BCCJ5337
BCCH9516
BCCJ4784

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E S Winn-Deen et al.
Clinical chemistry, 34(10), 2005-2008 (1988-10-01)
We describe a direct colorimetric assay for alpha-amylase, with 2-chloro-4-nitrophenyl-alpha-maltotrioside as substrate. Both human pancreatic and salivary amylase split this substrate without the use of helper enzymes, yielding free 2-chloro-4-nitrophenol, which is monitored at 405 nm. The performance of this
Kinetic determination of amylase on microtiter plates: an improved substrate.
J Laine et al.
Pancreas, 13(2), 217-217 (1996-08-01)
alpha-Amylase and alpha-glucosidase inhibitory saponins from Polyscias fruticosa leaves
Hanh TTH, et al.
Journal of Chemistry (2016)
A Y Foo et al.
Clinica chimica acta; international journal of clinical chemistry, 272(2), 137-147 (1998-06-26)
The use of 2-chloro-4-nitrophenyl maltotrioside (CNP-G3) as substrate to measure amylase (EC 3.2.1.1) activity in serum directly without the use of auxiliary enzymes was evaluated at two centres. The method was precise (within-run C.V. < 2% and between-run C.V. <
Klaus Lorentz
Clinical chemistry and laboratory medicine, 40(8), 781-785 (2002-10-24)
The advanced knowledge on substrate cleavage by human alpha-amylases promotes the development of chromogenic maltotriosides exclusively cleaved at the aglycone bond. Three essentials are required for this type of binding at the active site of the enzyme: (i) A minimal
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