72485

Nile Red

for microscopy

• Synonym(s): Nile Blue A Oxazone
• Empirical Formula (Hill Notation): C₂₀H₁₈N₂O₂
• CAS Number: 7385-67-3
• Molecular Weight: 318.37
• Beilstein: 279110
• EC Number: 230-966-0
• MDL number: MFCD00011639
• UNSPSC Code: 12171500
• PubChem Substance ID: 57652419
• NACRES: NA.32

Properties

• grade: for microscopy
• form: crystals
• mp: 203-205 °C (lit.)
• λmax: 553 nm
• SMILES string: CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34
• InChI: 1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
• InChI key: VOFUROIFQGPCGE-UHFFFAOYSA-N

Description

General description

Nile Red is a phenoxazone dye. It is an important stain, which is used to detect intracellular lipid droplets and proteins. Nile Red detects lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells. It exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.

Application

Nile Red has been used:

• as a fluorescent dye for staining fat by confocal scanning laser microscopy (CSLM)
• to label nanoparticles (NPs)
• for lipid analysis

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves