S7803
CpG WIZ™ MGMT - Methylation specific PCR assay
The components of the CpG WIZ MGMT Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.
Synonym(s):
MGMT Methylation Assay, Methylation PCR Kit
About This Item
Recommended Products
Quality Level
species reactivity
human
manufacturer/tradename
CPGWIZ™
Chemicon®
technique(s)
PCR: suitable
NCBI accession no.
application(s)
genomic analysis
Related Categories
General description
Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.
Application
Use of either the CpGenome DNA Modification Kit (Cat. No. S7820) or the CpGENOME Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ MGMT Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.
Components
U Primer Set5 μM each primer (25X) 35 μL (neutral cap) 90513 -15°C to -25°C
M Primer Set5 μM each primer (25X) 35 μL (red cap) 90514 -15°C to -25°C
W Primer Set5 μM each primer (25X) 35 μL (green cap) 90515 -15°C to -25°C
U control DNA0.1 μg/μL 50 μL (white cap) 90393 -15°C to -25°C
M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C
W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C
Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C
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Disclaimer
Storage Class Code
10 - Combustible liquids
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